ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (2): 225-232.doi: 10.11843/j.issn.0366-6964.2014.02.009

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Identification of Adipose-specific PU.1 Knockout Mice and Analysis of Their Live Body Fat and Lean Contents

PANG Wei-jun1,2*,WEI Ning1,WANG Yu1,XIONG Yan1,TONG Qiang2,YANG Gong-she1   

  1. (1.Laboratory of Animal Fat Deposition and Muscle Development,Northwest A & F University,Yangling 712100,China; 2.Baylor College of Medicine,Houston 77030,USA)
  • Received:2013-06-28 Online:2014-02-23 Published:2014-02-23

Abstract:

 aP2-cre-PU.1fl/fl mice were generated and identified using gene engineering,homologous recombination,PCR and Western blotting technologies in this study.The results showed that,compared with PU.1fl/fl mice,PU.1 protein was down-regulated to the level of that of aP2-cre-PU.1fl/fl.The body weight and body composition were analyzed in PU.1fl/fl and aP2-cre-PU.1fl/fl mice with 1-6 month old.Moreover,the body weight of aP2-cre-PU.1fl/fl mice was significantly higher than that of PU.1fl/fl mice at month 5 and month 6 (P<0.05).There was no significant difference in muscle weight but fat weight was significantly higher in aP2-cre-PU.1fl/fl mice than that in PU.1fl/fl mice (P<0.05).The results suggest that increase of adipose tissue of aP2-cre-PU.1fl/fl mice results in the increase of their body weight.Therefore,aP2-cre-PU.1fl/fl mice is the better animal model to explore PU.1 physiological functions in adipose tissue,which provide the theorical basis for exploring the effect of PU.1 gene on fat deposition in animals.

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